AI-supercharged BLAST nucleotide analysis & in silico primer probe verification in minutes, not weeks!

BLAST DeepSeq is an AI-powered automation tool for BLAST nucleotide alignment, inclusivity, exclusivity and variant analysis for molecular diagnostics & life science research.

What is Blast DeepSeq capable of?

Supercharge in-silico analysis & assay performance

  • Automate post-market surveillance and report generation for DNA/RNA assays.
  • Complete nucleotide BLAST alignments and variants analysis in minutes.
  • Intelligent primer-probe in silico analysis for inclusivity and exclusivity.
  • Accurate Primer and probe binding and qPCR performance prediction.

Automate primer-probe design verification & post-market surveillance

  • Sequence analysis of qPCR oligonucleotides to ensure applicability and relevance of your assay’s ability to detect the target(s) of interest (inclusivity) and to avoid any potential false positive detection (exclusivity).
  • BLAST DS can exclude your species of interest to specifically identify any potential cross-reactive species that may be detected by your assay.
  • Displays oligonucleotide binding locations across all accessions.
  • Provides alignment/homology information and includes primer-probe analysis to determine the assay's ability to amplify and detect a given accession.
  • Provides an overall detection rate based on the binding regions of your assay’s oligonucleotides to aid in empirical wet testing and optimization.

Accurate variant calling and annotation

  • Harness the power of advanced algorithms designed for highly accurate variant calling and annotation—pinpointing genetic variations, mutations, and sequence anomalies with confidence.
  • Identification of mutation/variant sequences present within the NCBI BLAST database for your amplicon/target of interest.
  • Results are automatically grouped into accessions with identical sequences, allowing quick identification and downloading of all accessions with a given mutation/variant.
  • Results are analyzed and parsed into detected and not detected accession groups, allowing quick identification and separation of accessions.

AI summary reports and results visualization

  • BLAST DS turns complex data into clarity with customizable reports that highlight your key findings.
  • Powerful visualization tools—ranging from sequence alignments to variant frequency plots and heatmaps—make it easier than ever to interpret results and share insights with your team.
  • BLAST DS results are analyzed and parsed into detected and not detected accession groups, allowing quick identification and separation of accessions.
  • Automated report generation showing inclusivity, exclusivity, and mutation/variant analysis.
Blast DeepSeq is trusted by.

Explore BLAST DeepSeq Output

The Description tab provides a detailed tabulation of the BLAST search results, offering a comprehensive overview of the identified sequences and their characteristics.
The Alignments tab provides detailed sequence alignments, displaying how the query sequence aligns with matched sequences from the BLAST search.
Groups together sequences having similar alignments and mutations. Sequences showing similar base chemistry are aligned and compressed together.
Groups together sequences having similar alignments and mutations. Sequences showing similar base chemistry are aligned and compressed together.
The Detection Groups tab categorizes accessions based on their detection status, grouping sequences as “Detected,” “Non-detected,” or requiring “Further Analysis.” This tab helps users quickly assess the outcome of organism detection.
The Summary Report tab provides Inclusivity in-silico analysis summary results.
The Mutation Analysis Report tab provides a Assessment of sequence variations (insertions, deletions, and mismatches) on assay specificity (inclusivity) and target detection.

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